binding-characterization

This skill provides guidance for optimizing cell-free protein synthesis (CFPS) experiments. It helps with planning, troubleshooting low yields, designing DNA templates, and expressing difficult proteins like membrane or disulfide-rich ones. Key features include a system selection guide comparing yields, PTMs, and costs for different CFPS platforms.

This skill provides guidance for planning and troubleshooting SPR and BLI binding kinetics experiments. It helps developers choose between platforms, interpret data artifacts, and resolve poor binding signals. Key features include a decision matrix comparing SPR/BLI and troubleshooting for common experimental issues.

This skill provides guidance for optimizing cell-free protein synthesis (CFPS) experiments. It helps developers plan experiments, troubleshoot low yields or aggregation, and design DNA templates, especially for difficult proteins like membrane or disulfide-rich ones. Key features include a system selection guide comparing yields, costs, and capabilities across different CFPS platforms.

This skill provides expert guidance for planning, troubleshooting, and interpreting Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI) binding kinetics experiments. It helps developers choose between SPR and BLI platforms using a detailed decision matrix and offers solutions for common issues like poor signal or data artifacts. Use it when designing or validating binding assays to ensure robust experimental results.