Mushroom Cultivation

Spawn → fruit, home scale.

Use When

• Want edible mushrooms, no wild forage risk
• Have indoor/outdoor space
• Try species + substrates
• Need reliable supply (food/medicine)
• Hands-on mycelium

In

• Required: Spawn (grain/sawdust/plug, reputable supplier)
• Required: Substrate (straw, hardwood sawdust, logs, supplemented sawdust)
• Optional: Pressure cooker / large pot (sterilize/pasteurize)
• Optional: Containers (bags, buckets, logs)
• Optional: Spray bottle + humidity gauge
• Optional: Thermometer

Do

Step 1: Pick species

Match env + skill.

Beginner-Friendly Species:
+--------------------+------------------+------------------+------------------+
| Species            | Substrate        | Temperature      | Difficulty       |
+--------------------+------------------+------------------+------------------+
| Oyster mushroom    | Straw, coffee    | 15-24C (60-75F)  | Very easy        |
| (Pleurotus spp.)   | grounds, sawdust |                  | (most forgiving) |
+--------------------+------------------+------------------+------------------+
| Shiitake           | Hardwood logs    | 13-21C (55-70F)  | Easy             |
| (Lentinula edodes) | or sawdust blocks|                  | (outdoor logs)   |
+--------------------+------------------+------------------+------------------+
| Lion's mane        | Hardwood sawdust | 18-24C (65-75F)  | Moderate         |
| (Hericium          | (supplemented)   |                  | (needs humidity) |
| erinaceus)         |                  |                  |                  |
+--------------------+------------------+------------------+------------------+
| Wine cap           | Wood chips,      | 10-27C (50-80F)  | Easy             |
| (Stropharia        | straw mulch      |                  | (outdoor beds)   |
| rugosoannulata)    | (outdoor beds)   |                  |                  |
+--------------------+------------------+------------------+------------------+

Start oyster — fast colonize, reliable fruit, forgiving.

→ Species fits env + substrate + skill.

If err: unsure → blue oyster (Pleurotus ostreatus) on straw.

Step 2: Prep substrate

Clean → mushroom beats competitors.

Substrate Preparation Methods:

PASTEURIZATION (for straw — easiest):
1. Chop straw to 2-4 inch lengths
2. Submerge in hot water (65-80C / 150-175F) for 60-90 minutes
3. Drain thoroughly — substrate should be moist but not dripping
   (squeeze test: a firm squeeze produces a few drops, not a stream)
4. Cool to below 30C (85F) before inoculation

STERILIZATION (for supplemented sawdust — more reliable):
1. Mix hardwood sawdust with 10-20% wheat bran or soy hull
2. Hydrate to 60-65% moisture content
3. Fill into autoclavable bags with filter patches
4. Pressure cook at 15 PSI for 90-120 minutes
5. Cool completely before inoculation (overnight is safest)

COLD WATER LIME BATH (alternative pasteurization):
1. Dissolve hydrated lime (calcium hydroxide) in cold water
   (approximately 1 cup per 50 gallons)
2. pH should reach 12+ (kills competitors without heat)
3. Soak straw for 12-18 hours
4. Drain and let excess water drip for 2-4 hours
5. pH will neutralize as the straw dries

→ Clean, right moisture, room temp.

If err: contam first week (green mold) → underpasteurized or dirty inoc. Restart, harder pasteurization.

Step 3: Inoculate

Spawn → substrate.

Inoculation Protocol:
1. Work in a clean environment: wash hands, clean surfaces, minimize airflow
   (still air is better than a breeze carrying contaminants)
2. Spawn rate: 10-20% spawn by weight relative to wet substrate
   (more spawn = faster colonization = less contamination risk)
3. Mix spawn thoroughly into the substrate (for bags/buckets)
   OR layer spawn between substrate layers
4. Pack into growing container:
   - Grow bags: fill loosely, fold and clip top
   - 5-gallon buckets: drill 1/2" holes in sides (every 6 inches),
     fill with inoculated substrate, cap loosely
   - Logs: drill holes, insert plug spawn, seal with wax
5. Label with species, date, and substrate type

Hygiene Priorities:
- Clean hands and surfaces
- Minimize time substrate is exposed to open air
- Work quickly and confidently
- If you touch a contaminated surface, re-clean before continuing

→ Spawn even, clean container, ready incubate.

If err: no white growth 7-10d → temp too cold / dry / dead spawn.

Step 4: Incubate

Mycelium colonizes.

Incubation Conditions:
+--------------------+------------------------------------------+
| Parameter          | Target                                   |
+--------------------+------------------------------------------+
| Temperature        | Species-specific (generally 20-25C /     |
|                    | 68-77F for most species)                 |
+--------------------+------------------------------------------+
| Light              | Dark or dim — direct light not needed    |
|                    | during colonization                      |
+--------------------+------------------------------------------+
| Air exchange       | Minimal — CO2 buildup is acceptable      |
|                    | during colonization (loose lid is enough)|
+--------------------+------------------------------------------+
| Duration           | 2-4 weeks (until substrate is fully      |
|                    | white with mycelium)                     |
+--------------------+------------------------------------------+
| Monitoring         | Check every 3-4 days for contamination   |
|                    | (green, black, orange, or pink mold)     |
+--------------------+------------------------------------------+

Contamination Response:
- Green mold (Trichoderma): most common competitor. If localized and
  small, remove the contaminated area. If widespread, discard the
  entire block/bag — Trichoderma wins once established.
- Black mold: discard immediately. Do not open indoors.
- Orange/pink: bacterial contamination from wet substrate. Discard.

→ Full white, mushroomy smell.

If err: partial + contam = race lost. Restart, more spawn, better pasteurize, cleaner work.

Step 5: Trigger fruit

Veg → mushroom.

Fruiting Triggers:
1. Fresh air: increase air exchange (open container, fan nearby)
2. Light: indirect light for 12 hours/day (any spectrum works)
3. Temperature drop: reduce by 5-10C from incubation temperature
4. Humidity: maintain 85-95% relative humidity
   - Mist 2-3 times daily
   - Or use a fruiting chamber (plastic tub with perlite floor)
5. For bags: cut X-shaped slits where you want mushrooms to emerge
   For buckets: mushrooms emerge from the drilled holes

Fruiting Chamber (Simple SGFC — Shotgun Fruiting Chamber):
- Large plastic storage tub (50-100L)
- Drill 1/4" holes every 2 inches on all 6 sides (including bottom and lid)
- 4-5 inch layer of wet perlite on the bottom
- Place colonized blocks/bags on a wire rack above the perlite
- Mist walls 2-3 times daily
- Fan fresh air in by waving the lid 2-3 times daily

→ Pins in 5-14d.

If err: no pins 2w → humidity (most common), light, temp too warm.

Step 6: Harvest + flush

Harvest Timing:
- Harvest just before or as the cap edges begin to flatten or turn upward
- For oysters: when the cap edges are still slightly curled downward
- For shiitake: when the cap is 70-80% open (partial veil still intact)
- For lion's mane: when spines are 0.5-1 cm long and still firm

Harvest Technique:
- Twist and pull gently at the base (preferred for most species)
- Or cut with a clean knife at the substrate surface
- Do not leave stumps that can rot and attract contamination

Successive Flushes:
- After harvesting, soak the block/bag in cold water for 12-24 hours
  (rehydration triggers the next flush)
- Return to fruiting conditions
- Expect 2-4 flushes, each smaller than the last
- Total yield: approximately 25-50% of wet substrate weight
  for oyster mushrooms over all flushes

→ Fresh mushrooms, optimal timing, flushes extend life.

If err: poor yield → depleted/contam. Supplemented = higher yield. Contam between flushes → done, compost.

Check

• Species fits env + skill
• Pasteurized/sterilized properly
• Spawn rate 10-20% wt
• Clean inoc
• Full colonize before fruit trigger
• Fruit conditions held
• Harvested optimal
• Flushes via rehydrate

Traps

• Bad pasteurize: top fail cause. Contam first week → inadequate
• Low spawn: slow colonize → competitors win. Use 10-20%
• Dry fruit: mushrooms 90% water. Dry → pins abort. <80% RH = too low
• No fresh air: high CO2 → long thin stems, small caps. Increase exchange
• Late harvest: over-mature drops spores, short shelf. Harvest early side
• Contam panic: small spot ≠ fatal. Isolate, remove, watch. Discard only if spreading

→

• fungi-identification — complementary; cultivation eliminates ID risk, but morphology helps spot contam
• prepare-soil — spent substrate = garden amendment; cycle connects to soil